Cat. No.
MABL-3816
Application
WB, ELISA, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
6F4
From
Recombinant Antibody
Specificity
This antibody specifically recognizes human junctional adhesion molecule A and does not cross-react with other members of the JAM family. JAM-A is an adherens and tight junction protein expressed by endothelial and epithelial cells. It is recently identified as an antigenic protein (Ag) overexpressed in tumor cells and associated with cancer progression.
Alternative Names
CD321; JAM-1; JAM1; JCAM; F11R; PAM-1; Junctional adhesion molecule A; Junctional adhesion molecule 1; Platelet F11 receptor
UniProt
Q9Y624
Immunogen
The original antibody was generated by immunizing BALB/c mice with MCF-7 cells. The humanized version was generated by grafting CDRs of parental mouse antibody onto human framework regions.
Application Notes
This antibody was used for the characterization of human junctional adhesion molecule-A (JAM-A) and study of its expression on various cancerous tissues. The binding specificity of this antibody for human JAM-A expressed on CHO cells was confirmed using flow cytometry. JAM-A expression level on cell surface was determined by immunofluorescence staining and visualized by confocal microscopy. This antibody can also be used in the determination of JAM-A protein using western blot.Immunohistochemical analysis using this antibody revealed that JAM-A is overexpressed on breast, lung and kidney tumor tissues. Injections of anti-JAM-A antibody resulted in a significant tumor growth inhibition of xenograft human tumors in vivo. Treatment with monoclonal antibody induced a decrease of the Ki67 expression and downregulated JAM-A levels. This antigen may play a role in interfering with tumor proliferation (PMID: 22886345). This antibody binds the extracellular domain of JAM-A in an ELISA (Goetsch et al, 2011).
Antibody First Published
Goetsch et al. A novel role for junctional adhesion molecule-A in tumor proliferation: modulation by an anti-JAM-A monoclonal antibody. Int J Cancer. 2013 Mar 15;132(6):1463-74. PMID:22886345
Note on publication
Describes the characterization of a new target expressed on cancerous tissues using this antibody
Size
297 μg Purified antibody.
Concentration
198 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 497.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 217⁰C.
![Anti-VEGF [Bevacizumab] (MABL-](/public/static/common/images/not_adv.jpg)
