Cat. No.
MABL-3817
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
D113
From
Recombinant Antibody
Specificity
This antibody binds the G12D mutant form of human KRAS protein. This ras protein binds GDP/GTP and possess intrinsic GTPase activity, that results in conversion of nucleotide guanosine triphosphate (GTP) into guanosine diphosphate (GDP). This protein plays an important role in many signal transduction pathways and regulation of cell proliferation. Somatic KRAS mutations are highly observed in leukemias, colorectal cancer, pancreatic cancer and lung cancer. KRAS mutation is G12D which is estimated to be present in up to 37% pancreatic cancers and over 12% of colorectal cancers.
Alternative Names
K-Ras; GTPase Kras; KRAS2; RASK2; K-Ras 2; Ki-Ras; c-K-ras; c-Ki-ras; ras p21 protein; p21 ras; D113-2-1
UniProt
P01116-2
Immunogen
The original antibody was generated by immunizing mice with a synthetic dodecapeptide 'KLVVVGADGVGK'.
Application Notes
The binding reactivity and epitope characterization of this antibody towards human KRAS G12D mutant was determined using ELISA (PMID: 9440050). This antibody can bind both the peptide 'KLVVVGADGVGK' conjugated BSA as well as KRAS G12D mutant in an ELISA.
Antibody First Published
Wang et al. Epitopic characterization of the human wild-type and mutant ras proteins using membrane-bound peptides. J Pept Res. 1997 Dec;50(6):483-92. PMID:9440050
Note on publication
Describes the epitope characterization of many anti-ras protein antibodies.
Size
298 μg Purified antibody.
Concentration
199 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 498.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 218⁰C.
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