Cat. No.
MABL-3818
Application
humanisation, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
3S193
From
Recombinant Antibody
Specificity
Lewis Y (Ley) is a blood group antigen expressed at high levels on the surface of epithelial tumors, including small cell lung cancer.
Alternative Names
Ley
UniProt
Immunogen
3S193 (IgG3) was generated in BALB/c mice by immunisation with Ley-expressing cells of the MCF-7 breast carcinoma cell-line.
Application Notes
The murine 3S193 showed high specificity for Ley in ELISA tests with synthetic Ley and Ley-containing glycoproteins and glycolipids and also reacted strongly in rosetting assays and cytotoxic tests with Ley-expressing cells (Scott et al, 2000). Murine 3S193 was humanised and in antibody-dependent cell-mediated cytotoxicity assays displayed 100-fold greater activity (Kitamura et al, 1994). 3S193 has been used to screen tumour samples for Ley expression using immunohistochemistry (Krug et al, 2007). A crystal structure of the humanised antibody has been obtained to 1.9 Å resolution (Ramsland et al, 2004).
Antibody First Published
Kitamura et al, 1994. Specificity analysis of blood group Lewis-y (Le(y)) antibodies generatedagainst synthetic and natural Le(y) determinants. Proc Natl Acad Sci U S A. 1994 Dec 20; 91(26): 12957–12961 PMID:7809154
Note on publication
Describes the original generation of the antibody and its application to ELISA.
Size
299 μg Purified antibody.
Concentration
200 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 499.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 219⁰C.
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