Cat. No.
MABL-3819
Application
ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
BR96
From
Recombinant Antibody
Specificity
The antibody binds Le(Y) with a Ka of 0.1 μM.
Alternative Names
Lewis Y
UniProt
Immunogen
H3396 cultured line established from a metastasis of a human breast adenocarcinoma.
Application Notes
The antibody binds specifically to LeY tetrasaccharide, a carbohydrate antigen expressed at the cell surface of selective carcinomas, including those of the colon, breast, ovary and lung. Studies have shown that the monoclonal antibody has toxic effects to antigen-positive tumour cells, performing ADCC at concentrations down to 0.1 μg/ml. The antibody B396 was also shown to perform complement-dependent cytotoxicity at concentrations of 0.1-5.0 μg/ml in certain carcinoma cell lines. The monoclonal antibody also has the ability to be internalised by antigen-positive tumour cells, and can thereby be used to deliver anti-cancer drugs to the specific tumour cells.
Antibody First Published
Hellström I, Garrigues HJ, Garrigues U, Hellström KE. Highly tumor-reactive, internalizing, mouse monoclonal antibodies to Le(y)-related cell surface antigens. Cancer Res. 1990 Apr 1;50(7):2183-90. PMID:1690595
Note on publication
Describes the generation of two monoclonal antibodies, BR64 and BR69, which are selective for cell surface antigens present in certain carcinomas. The antibodies are internalised by antigen- positive tumour cells.
Size
300 μg Purified antibody.
Concentration
201 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 500.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 220⁰C.
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