Cat. No.
MABL-3849
Application
in vitro, IP, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
8C12
From
Recombinant Antibody
Specificity
The antibody is specific for PSMA. The antibody recognises a conformational epitope.
Alternative Names
PSM; FOLH1; FOLH; FGCP; GCPII; mGCP; NAALAD1; GIG27; Prostate specific membrane antigen; Glutamate carboxypeptidase 2; Cell growth-inhibiting gene 27 protein; Folate hydrolase 1; Folylpoly-gamma-glutamate carboxypeptidase; Glutamate carboxypeptidase II; Membrane glutamate carboxypeptidase; N-acetylated-alpha-linked acidic dipeptidase I; NAALADase I; Prostate-specific membrane antigen; Pteroylpoly-gamma-glutamate carboxypeptidase
UniProt
Q04609
Immunogen
The original antibody was generated by immunizing transgenic mice with PSMA.
Application Notes
The specificity of the antibody for PSMA was determined by ELISA analysis. The ability of the original format of the antibody and the bispecific 14A8 x 8C12 antibody to bind to PSMA-expressing LNCaP cells and CD89-expressing U937 cells was evaluated by solid phase ELISA. The antibody demonstrated high specificity for LNCaP cell membranes over a range of antigen concentrations, while the bispecific molecule binds both LNCaP and U937 cells in a dose-dependent fashion. The original format of the antibody immunoprecipitated PSMA from LNCaP Cells. This antibody was used for detection of PSMA expressed on LNCaP cells by flow cytometry. The original format and the bispecific 14A8 x 8C12 antibodies were tested for cell- mediated ADCC killing of labeled PSMA-expressing tumor cells. The results showed that both versions mediated dose dependent lysis by monocytes of LNCaP tumor cells. The original format and the bispecific 14A8 x 8C12 were tested for their ability to mediate phagocytosis of labeled PSMA-expressing tumor cells (LNCaP cells). Results showed that both were able to mediate phagocytosis of LNCaP cells in a dose dependent fashion (WO2003064606A3).
Antibody First Published
Note on publication
Size
330 μg Purified antibody.
Concentration
231 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 530.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 250⁰C.
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