Cat. No.
MABL-004
Application
immunoblot, IP, WB, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Rat, Human, Mouse
Clone No.
735
From
Recombinant Antibody
Specificity
Recognises Polysialic acid (PSA), a carbohydrate polymer attached to the neural cell adhesion molecule (NCAM), which shares molecular epitopes with homopolymers of α2,8-linked sialic acid of Escherichia coli K1.
Alternative Names
PSA; N-CAM; neural cell adhesion molecule; polySA
UniProt
Immunogen
Homopolymers of α2,8-linked sialic acid of Escherichia coli K1.
Application Notes
Clone 735 was used for immunostaining and immunoprecipitation in an experimental
setup involving antigen preparation from PBMC (Peripheral Blood Mononuclear Cells) (Husmann et al.,
1989; PMID: 2529126). This antibody recognises an unusual postranslational modification on neural cell
adhesion molecules important in synaptogenesis, oncogenesis (particularly small-cell lung cancer) and
development of organs such as the kidney (studied in rats, Lackie et al., 1990). 735 was used for Western
blot analysis of PSA-NCAM (Polysialylated Neural Cell Adhesion Molecule) (Charter et al., 2000; PMID:
11030751). In addition to various studies on human cells, the antibody, has also been used the study the
fine-composition of poly-sialic acid composition in mice (Galuska et al., 2006).
Antibody First Published
Bitter-Suermann & Roth Monoclonal antibodies to polysialic acid reveal
epitope sharing between invasive pathogenic bacteria, differentiating cells and tumor cells. Immunol Res.
1987;6(4):225-37. PMID:2448401
Note on publication
Describes the generation of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
