Cat. No.
MABL-050
Application
IP, neutralising, ELISA
Isotype
Engineer antibody
Species Reactivity
Cow, Sheep, Mouse
Clone No.
6H10
From
Recombinant Antibody
Specificity
This antibody is specific for PrPSc, and binds to a conformational epitope that incudes the extreme C-terminus of PrP. It does not react with recombinant mouse PrP or denatured PrPSc.
Alternative Names
Prion; prion protein; PrP; CD230; PRNP; PRP; SIP
UniProt
P23907
Immunogen
The binding specificity of this antibody for PrPSc has been confirmed in ELISA analysis; this antibody reacts with non-denatured PrPSc, but not with recombinant mouse PrP or denatured PrPSc treated with more than 3 M GdnHCl (Horiuchi et al, 2009). This antibody has been shown to immunoprecipitate PrPSc from the brains of mice, sheep, and cattle infected with prions (Horiuchi et al, 2009). Pretreatment of purified PrPSc with this antibody results in a 1 log reduction in infectious titre (Horiuchi et al, 2009).This antibody has also been used to identify C-terminal conformational differences between prion strains (Saijo et al, 2016).As this antibody recognises a conformational epitope on PrPSc, it does not react with PrPSc in immunoblot analysis (Horiuchi et al, 2009).
Application Notes
The binding specificity of this antibody for PrPSc has been confirmed in ELISA analysis; this antibody reacts with non-denatured PrPSc, but not with recombinant mouse PrP or denatured PrPSc treated with more than 3 M GdnHCl (Horiuchi et al, 2009). This antibody has been shown to immunoprecipitate PrPSc from the brains of mice, sheep, and cattle infected with prions (Horiuchi et al, 2009). Pretreatment of purified PrPSc with this antibody results in a 1 log reduction in infectious titre (Horiuchi et al, 2009).This antibody has also been used to identify C-terminal conformational differences between prion strains (Saijo et al, 2016).As this antibody recognises a conformational epitope on PrPSc, it does not react with PrPSc in immunoblot analysis (Horiuchi et al, 2009).
Antibody First Published
Horiuchi et al. Generation of monoclonal antibody that distinguishes PrPSc from PrPC and neutralizes prion infectivity Virology; Volume 394, Issue 2, 25 November 2009, Pages 200-207 PMID:19766283
Note on publication
Describes the original generation of this antibody, and its characterisation.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
