Cat. No.
MABL-052
Application
Blocking, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Rat, Human
Clone No.
M38
From
Recombinant Antibody
Specificity
von Budingen et al Molecular characterization of antibody specificities against myelin/oligodendrocyte glycoprotein in autoimmune demyelination Proc. Natl. Acad. Sci. U.S.A. 99 (12), 8207-8212 (2002)
Alternative Names
Myelin oligodendrocyte glycoprotein; rMOG
UniProt
Q63346
Immunogen
Callithrix jacchus marmosets were used, and experimental allergic encephalomyelitis was induced by the injection of rat MOG (aa1-125) into the marmosettes. The rMOG was expressed in E. coli and purified to homogeneity. The animals were killed 4-70 days after the onset of symptoms of EAE. Bone marrow and spleen cells were obtained from an immunized C. jacchus, the RNA extracted with Trizol reagent and rtPCR used to generate cloning inserts containing Fab portions of IgGk. Phage display was then used to select for the MOG-reactive Fab fragments using the pCOMB3H phage display vector, and binding confirmed using an ELISA.
Application Notes
Recognises a similar epitope as M26 and M45 as they have been shown to compete for binding. Also competes with M3-8 and so is likely to have overlapping epitopes. Recognises an epitope distinct from M3-31 or M2-24. The epitope recognised is a structural epitope, shown by the lack of recognition of linear MOG peptides.
Antibody First Published
von Budingen et al Molecular characterization of antibody specificities against myelin/oligodendrocyte glycoprotein in autoimmune demyelination Proc. Natl. Acad. Sci. U.S.A. 99 (12), 8207-8212 (2002) PMID:12060766
Note on publication
An analysis of 6 Fab fragments against epitopes on MOG, identifying at least 4 separate epitopes. Analysis of these epitopes including competition assays with marmoset auto-antibodies,human auto-antibodies from MS patients, and confirmation that the epit
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
