Cat. No.
MABL-056
Application
Blocking, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Rat, Human
Clone No.
M3-31
From
Recombinant Antibody
Specificity
Is displaced by M38 and M45 and is weakly displaced by M26 although this is not mutual which may suggest the M26-mediated displacement is conformationally driven, although this has not been confirmed. The epitope recognised is a structural epitope, shown by the lack of recognition of linear MOG peptides. Binds MOG expressed in CHO cells.
Alternative Names
Myelin oligodendrocyte glycoprotein; rMOG; hMOG
UniProt
Q63350
Immunogen
Callithrix jacchus marmosets were used, and experimental allergic encephalomyelitis was induced by the injection of rat MOG (aa1-125) into the marmosettes. The rMOG was expressed in E. coli and purified to homogeneity. The animals were killed 4-70 days after the onset of symptoms of EAE. Bone marrow and spleen cells were obtained from an immunized C. jacchus, the RNA extracted with Trizol reagent and rtPCR used to generate cloning inserts containing Fab portions of IgGk. Phage display was then used to select for the MOG-reactive Fab fragments using the pCOMB3H phage display vector, and binding confirmed using an ELISA.
Application Notes
This antibody has been proposed for the diagnosis and prognosis of multiple sclerosis (MS) or allergic encephalomyelitis (EAE) by using competition assays to determine if there are autoantibodies present in an individual. It has also been proposed that it could be used to predict the severity of the MS or EAE by detecting the proportion of conformation-specific autoantibodies to linear epitope autoantibodies. This antibody has been used in ELISAs and competition assays to characterise its epitope in relation to other antibodies generated against MOG. It was originally generated and tested as a Fab fragment (vod Budingen et al, 2002). This antibody has also shown to bind to hMOG expressed in a CHO cell line (Lalive et al, 2006). Analysis has also been done on the amino acid sequence of this antibody (von Budingen et al, 2006).
Antibody First Published
von Budingen et al Molecular characterization of antibody specificities against myelin/oligodendrocyte glycoprotein in autoimmune demyelination Proc. Natl. Acad. Sci. U.S.A. 99
(12), 8207-8212 (2002) PMID:12060766
Note on publication
An analysis of 6 Fab fragments against epitopes on MOG, identifying at least 4 separate epitopes. Analysis of these epitopes including competition assays with marmoset auto-antibodies, human auto-antibodies from MS patients, and confirmation that the epit
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
