Cat. No.
MABL-071
Application
IB, IP, WB, IF
Isotype
Engineer antibody
Species Reactivity
Rat, Human, Mouse
Clone No.
K28/43
From
Recombinant Antibody
Specificity
This antibody is specific for PSD-95. It has been shown not to cross-react with DLG1/SAP97, DLG2/Chapsyn-110 or DLG3/SAP102.
Alternative Names
K28/43R; pSD95; Postsynaptic density protein 95; Disks large homolog 4; Synapse-associated protein 90; DLG4; Dlgh4; SAP-90
UniProt
P78352
Immunogen
This antibody was raised by immunising BALB/c mice with a fusion protein consisting of amino acids 77-299 (PDZ domains 1 and 2) of human PSD-95.
Application Notes
This antibody has been used in immunoblot analysis of membranes prepared from adult rat and mouse brain (Andrews et al, 2019; PMID:30667360; Rasband et al, 2019). This antibody has also been used in immunofluorescence staining of cultured rat hippocampal neurons and immunohistochemical analysis of adult rat brain sections (Andrews et al, 2019; PMID:30667360).Additionally, this antibody has been used in western blot analysis of rat spinal dorsal horn neurons (Li et al, 2019) and immunogold electron microscopy (Andrews et al, 2019; PMID:30667360).
Antibody First Published
Rasband et al. Clustering of neuronal potassium channels is independent of their interaction with PSD-95 J Cell Biol. 2002;159(4):663‐672 PMID:12438413
Note on publication
Describes the original generation of this antibody and its initial characterisation.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
