Cat. No.
MABL-134
Application
ICC, WB, IF
Isotype
Engineer antibody
Species Reactivity
Rat, Torpedo californica, Mouse
Clone No.
N34/34.2
From
Recombinant Antibody
Specificity
This antibody is specific for Vglut3. Vglut3 mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.
Alternative Names
N34/34.2R; N34/34; Vesicular glutamate transporter 3; Slc17a8; Solute carrier family 17 member 8
UniProt
Q7TSF2
Immunogen
This antibody was raised by immunising BALB/c mice with a fusion protein amino acids 546- 588 (cytoplasmic C-terminus) of rat VGlut3.
Application Notes
The IgG1 version of this antibody has been used for immunocytochemistry on a peptide corresponding to the antigen (Fyk-Kolodziej et al, 2011; PMID: 21211553). The IgG1 version of this antibody has been used for western blotting on the DCN and VCN of deaf mice (Fyk-Kolodziej et al, 2011; PMID: 21211553). Immunofluorescense on PVCN of rats has been preformed by using the IgG1 verion of this antibody (Fyk-Kolodziej et al, 2011; PMID: 21211553). The IgG1 version of this antibody was used to preform western blot and immunofluorescense on the electroplaque of Torpedo californica (Li and Harlow, 2014; PMID:24744885).
Antibody First Published
Andrews et al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain eLife. 2019; 8: e43322. PMID:30667360
Note on publication
This article describes the generation of a library of recombinant monoclonal antibodies (R-mAbs) from a pool of mAb-producing hybridomas for neuroscience research.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
