Key features and details | |
Cat. No. | MABL-004 |
Name | Anti-Polysialic acid mAbs |
Clone No. | AFD-735 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | immunoblot, IP, WB, FC, IHC |
Species Reactivity | Rat, Human, Mouse |
Basic Information | |
Specificity | Recognises Polysialic acid (PSA), a carbohydrate polymer attached to the neural cell adhesion molecule (NCAM), which shares molecular epitopes with homopolymers of α2,8-linked sialic acid of Escherichia coli K1. |
Alternative Name | PSA; N-CAM; neural cell adhesion molecule; polySA |
UniProt | |
Immunogen | Homopolymers of α2,8-linked sialic acid of Escherichia coli K1. |
Application Notes | Clone 735 was used for immunostaining and immunoprecipitation in an experimental setup involving antigen preparation from PBMC (Peripheral Blood Mononuclear Cells) (Husmann et al.,1989; PMID: 2529126). This antibody recognises an unusual postranslational modification on neural cell adhesion molecules important in synaptogenesis, oncogenesis (particularly small-cell lung cancer) and development of organs such as the kidney (studied in rats, Lackie et al., 1990). 735 was used for Western blot analysis of PSA-NCAM (Polysialylated Neural Cell Adhesion Molecule) (Charter et al., 2000; PMID: 11030751). In addition to various studies on human cells, the antibody, has also been used the study the fine-composition of poly-sialic acid composition in mice (Galuska et al., 2006). |
Antibody First Published | Bitter-Suermann & Roth Monoclonal antibodies to polysialic acid reveal epitope sharing between invasive pathogenic bacteria, differentiating cells and tumor cells. Immunol Res.1987;6(4):225-37. PMID:2448401 |
Note on publication | Describes the generation of this antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
