Anti-CD40L [MR1](MABL-3494)

Cat. No.

MABL-3494

Name

Anti-CD40L [MR1](MABL-3494)

Clone No.

MR1

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Blocking, functional assays, FC

Species Reactivity

Mouse

Basic Information

Specificity

This antibody is specific for murine CD40   ligand (CD40L), a 39 kDa transmembrane glycoprotein. CD40L is expressed   transiently by activated T cells. Through its binding to CD40 on antigen   presenting cells (APC) including B cells, monocytes/macrophages, and   dendritic cells, it serves a crucial function in T cell-APC cognate   interaction. CD40L-interaction with CD40 transduces signals for T-dependent B   cell activation and induces B cells to enter the cell cycle.

Alternative Name

CD154; CD40 ligand; CD40-L; Cd40l; gp39; HIGM1;   IGM; IMD3; Ly-62; Ly62; RP23-153G22.3; T-BAM; T-cell antigen Gp39;   TNF-related activation protein; Tnfsf5; TRAP; Tumor necrosis factor ligand   superfamily member 5.

UniProt

P27548

Immunogen

This antibody was raised by immunising Armenian hamsters with murine activated Th1 (D1.6) plasma membrane as   described by Noelle et al (1992).

Application Notes

This antibody was administred to SOD1 G93A   mice, which exhibit ALS like symptoms, it was able to delay paralysis onset, it improved body-weight maintenance and extended survival (Lincecum et al.,   2010; PMID:20348957). This antibody has been used in various FACS analyses   for diverse immunological applications, such as to indicate how naive CD4 T   cells constitutively express CD40L and augment autoreactive B cell survival   (Lesley et al, 2006), to prove that the interaction between natural killer   cells and dendritic cells has a pivotal role in the sensitization phase of   contact hypersensitivity (Shimizuhira et al, 2014), and to demonstrate that   enhanced CD8 T cell responses through GITR-mediated costimulation could   resolve chronic viral infection (Pascutti et al, 2015). This antibody has   also been used for in vitro and in vivo blocking and functional studies, for   instance, to indicate that the 39-kDa CD40L membrane protein expressed on   activated Th is a binding protein for CD40 and functions to transduce the signal   for Th- dependent B-cell activation (Noelle et al, 1992), and to demonstrate   that the CD40L–CD40 pathway can augment the survival of autoantigen-engaged B   cells in the absence of T cell activation (Lesley et al, 2006).

Antibody First   Published

Noelle et al. A 39-kDa protein on activated   helper T cells binds CD40 and transduces the signal for cognate activation of   B cells. Proc Natl Acad Sci U S A. 1992 Jul 15;89(14):6550-4. PMID:1378631

Note on publication

Describe the original generation of this   antibody and its subsequent use in FACS, blocking and functional studies to   indicate that the 39-kDa membrane protein expressed on activated Th is a binding protein for CD40 and functions to transduce the signal for   Th-dependent B-cell activation.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.