Key features and details | |
Cat. No. | MABL-024 |
Name | Anti-Y108F-LeuT mAbs |
Clone No. | AFD-2B12 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | FSEC, WB |
Species Reactivity | Aquifex aeolicus (strain VF5) |
Basic Information | |
Specificity | / |
Alternative Name | Bacterial Leucine Transporter |
UniProt | O67854 |
Immunogen | Purified LeuT in detergent. |
Application Notes | The antibody binds specifically to LeuT is a bacterial symporter specific for small hydrophobic amino acids such as leucine and alanine, driven by the sodium electrochemical gradient across the membrane. The antibody is specific for the outward-open conformation (substrate-free/ sodium- bound states) of LeuT, which is stabilised by mutating Tyr108 to Phe in TM3 (Y108F_LeuT). The 2B12 antibody was selected to bind to this conformational state by fluorescence-detection size-exclusion chromatography (FSEC) and western Blot. The Fab fragment of the antibody was obtained by papain digestion. LeuT is a bacterial symporter specific for small hydrophobic amino acids such as leucine and alanine, driven by the sodium electrochemical gradient across the membrane. Due to the high homology of LeuT to human neurotransmitter transporters, its crystal structure can reveal the mechanism of action of many drugs, such as antidepressants. |
Antibody First Published | Krishnamurthy et al. X-ray structures of LeuT in substrate-free outward- open and apo inward-open states. Nature. 2012 Jan 9;481(7382):469-74. PMID:22230955 |
Note on publication | Describes the role of neurotransmitter sodium symporters (NSS) in chemical neurotransmission, and the importance of LeuT, a bacterial NSS homologue, as a structural template. Crystal structures of the outward-open and inward-open states of LeuT, stabilised by the monoclonal antibodies 2B12 and 6A10, are presented. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
