Key features and details | |
Cat. No. | MABL-075 |
Name | Anti-Kv4.2 K+ Channel Ectodomain mAbs |
Clone No. | AFD-K57/1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IB, IP, IHC |
Species Reactivity | C. elegans, Rat, Mouse |
Basic Information | |
Specificity | This antibody is specific for the ectodomain of the Kv4.2 potassium channel. It does not react with Kv4.3 and Kv4.1. |
Alternative Name | K57/1R; Potassium voltage-gated channel subfamily D member 2; Voltage-gated potassium channel subunit Kv4.2; Kcnd2; Kiaa1044; MNCb-7013; RK5; Shal1 |
UniProt | Q9NZV8 |
Immunogen | This antibody was raised by immunising BALB/c mice with a synthetic peptide corresponding to the extracellular S1-S2 loop (amino acids 209-225) of human Kv4.2, where the third Ser was substituted in place of a Cys for peptide conjugation purposes. |
Application Notes | This antibody has been used in immunoblot analysis of crude membranes from rat and mouse brain (Andrews et al, 2019; PMID:30667360), as well as crude C. elegans extracts (Andrews et al, 2019; PMID:30667360) and lysates of COS-1 cells transfected to express Kv4.2 (Shibata et al, 2003). It has also been used in immunohistochemical analysis of adult rat and mouse brain sections (Sack et al, 2013; Andrews et al, 2019; PMID:30667360), and immunofluorescence analysis of surface Kv4.2 expression by Kv4.2-transfected COS-1 cells (Shibata et al, 2003). Using ELISA, pre-incubation with soluble Kv4.2 peptide has been shown to block the binding of this antibody to its antigen (Sack et al, 2013). This antibody, when labelled with gold, has been used in electron microscopy to assess endogenous Kv4.2 expression in rat hippocampal sections (Kim et al, 2007). |
Antibody First Published | Shibata et al. A Fundamental Role for KChIPs in Determining the Molecular Properties and Trafficking of Kv4.2 Potassium Channels. J Biol Chem. 2003;278(38):36445‐36454.PMID:12829703 |
Note on publication | Describes the original generation of this antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
