Anti-Pan-Nav channel mAbs

Cat. No.

MABL-076

Name

Anti-Pan-Nav channel  mAbs

Clone No.

AFD-K58/35

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IB, WB, IHC

Species Reactivity

Vertebrates

Basic Information

Specificity

This pan-voltage-gated sodium channel   antibody is specific for a conserved sequence present in all vertebrate Nav1   isoforms. It binds to all voltage-gated sodium channel isoforms.

Alternative Name

K58/35R; sodium voltage-gated   channel; Na+ channel

UniProt

Immunogen

This antibody was raised by   immunising mice with a synthetic peptide corresponding to a highly conserved   segment of the intracellular III–IV loop of vertebrate Na+ channels.

Application Notes

This antibody has been used to stain   teased axons prepared from rat spinal nerves (Devaux, 2012), sections of rat   retina (Wart et al, 2005) and mice optic nerve cryosections (Vega et al,   2008) for Nav expression. This antibody has been shown to label Nav channels   concentrated on the axon initial segment and nodes of Ranvier (Andrews et al,   2019). This antibody has been used in immunoblot analysis of rat brain   membranes (Wart et al, 2005; Vega et al, 2013), and to stain COS-1 cells   transfected with Nav1 cDNA (Wart et al, 2005). Additionally, this antibody   has been used in western blot analysis of mice optic nerve and brain membrane   proteins (Vega et al, 2008).

Antibody First Published

Rasband et al. Dependence of Nodal   Sodium Channel Clustering on Paranodal Axoglial Contact in the Developing CNS   Journal of Neuroscience 1 September 1999, 19 (17) 7516-7528; PMID:10460258

Note on publication

Describes the original generation of   this antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.