Key features and details | |
Cat. No. | MABL-077 |
Name | Anti-SAP97 scaffold protein mAbs |
Clone No. | AFD-K64/15 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IB, IHC, IP |
Species Reactivity | Rat, Mouse |
Basic Information | |
Specificity | This antibody is specific for SAP97. It does not cross-react with PSD-95, Chapsyn-110 and SAP102. |
Alternative Name | K64/15R; synapse-associated protein 97; disks large homolog 1; SAP-97; DLG1; Q811D0 |
UniProt | Q62696 |
Immunogen | This antibody was raised by immunising BALB/c mice with a fusion protein corresponding to the N-terminus (amino acids 1-104) of rat SAP97. |
Application Notes | This antibody has been used in immunoblot analysis of rat (Hallaq et al, 2007) and mouse brain membranes (Andrews et al, 2019; PMID:30667360). It has also been used in immunohistochemical analysis of adult rat hippocampus sections (Andrews et al, 2019; PMID:30667360), as well as western blot analysis of mouse hippocampus synaptoneurosomes and proteins (Freudenberg, 2019). Additionally, this antibody has been used in immunoprecipitation analysis of rat cerebral cortical tissue (von Ossowski et al, 2017). |
Antibody First Published | Hallaq et al. NMDA Di-Heteromeric Receptor Populations and Associated Proteins in Rat Hippocampus The Journal of Neuroscience, August 1, 2007 27(31):8334 – 8343 PMID:17670980 |
Note on publication | Describes the original use of this antibody in immunoprecipitation analysis. |
COA Information (For reference only, actual COA shall prevail) | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |