Anti-Mitofusin-2 mAbs

Cat. No.

MABL-101

Name

Anti-Mitofusin-2 mAbs

Clone No.

AFD-N153/5.1

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ICC, WB, IF

Species Reactivity

Mouse

Basic Information

Specificity

 This antibody is specific for the mitofusion-2 protein and shows no   cross reactivity with mitofusion-1. Mitofusion-2 is a mitochondrial outer   membrane GTPase that mediates mitochondrial clustering and fusion

Alternative Name

N153/5.1R; N153/5;   Hypertension-related protein 1; Mitochondrial assembly regulatory factor; HSG   protein; Transmembrane GTPase MFN2

UniProt

 Q80U63

Immunogen

This antibody was raised by   immunising BALB/c mice with a fusion protein corresponding to Mouse Mitofusin   2 aa 370-600.

Application Notes

 This antibody is recommended for the detection and analysis of   mitofusin-2 by western blot, immunofluorescence and immunocytochemistry. For   instance, the mouse version of this antibody was used to detect Mitofusin-2   by western blot in extract of crude cardiac mitochondrial preps from WT, Mfn1   KO or Mfn2 KO mice. It was also used for immunohistochemistry in which   Mitofusin-2 was detected in adult rat hippocampus.

Antibody First Published

Andrews et al. A toolbox of IgG   subclass-switched recombinant monoclonal antibodies for enhanced multiplex   immunolabeling of brain eLife. 2019; 8: e43322. PMID:30667360

Note on publication

This article describes the   generation of a library of recombinant monoclonal antibodies (R-mAbs) from a   pool of mAb-producing hybridomas for neuroscience research.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.