Key features and details | |
Cat. No. | MABL-103 |
Name | Anti-Navbeta4 Na+ channel mAbs |
Clone No. | AFD-N168/6.3 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ICC, WB, IHC |
Species Reactivity | Rat, Mouse |
Basic Information | |
Specificity | This antibody is specific for the Navbeta4 protein in mice and rats. This protein Modulates channel gating kinetics. |
Alternative Name | N168/6.3R; N168/6; Sodium channel subunit beta-4; Scn4b; Navbeta4 |
UniProt | Q7M730 |
Immunogen | This antibody was raised by immunising BALB/c mice with a fusion protein amino acids 184- 228 (cytoplasmic C-terminus) of rat Navbeta4. |
Application Notes | This antibody is recommended for the detection and analysis of Navbeta4 Na+ channel by immunocytochemistry, western blot and immunohistochemistry. For instance, the mouse version of this antibody was used to detect Navbeta4 Na+ channel by western blot in extracts of RBM and COS cells transiently transfected with GFP-tagged Navbeta4 or untagged Kv2.1 plasmid. Furthermore it was used to detect Navbeta4 Na+ channel by immunocytochemistry in adult rat sciatic nerves. And finally it was also used for detection of Navbeta4 Na+ channel by immunohistochemistry in adult rat brain. |
Antibody First Published | Andrews et al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain eLife. 2019; 8: e43322. PMID:30667360 |
Note on publication | This article describes the generation of a library of recombinant monoclonal antibodies (R-mAbs) from a pool of mAb-producing hybridomas for neuroscience research. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
