Anti-PARIS/ZNF746 mAbs

Cat. No.

MABL-111

Name

Anti-PARIS/ZNF746  mAbs

Clone No.

AFD-N196/16

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IP, WB, IHC

Species Reactivity

Rat, Human, Mouse

Basic Information

Specificity

This antibody is specific for the   PARIS/ZNF746 protein. The antibody does not cross-react with ZNF783 or   ZNF398. ZNF746 is a transcription repressor that specifically binds to the   5'-TATTTT[T/G]-3' consensus sequence on promoters and repress transcription   of PGC-1-alpha (PPARGC1A), thereby playing a role in regulation of neuron   death.

Alternative Name

N196/16R; Zinc finger protein 746; ZNF746;   Parkin-interacting substrate; PARIS

UniProt

Q6NUN9

Immunogen

This antibody was raised by immunising   BALB/c mice with a fusion protein amino acids 1-322 (N-terminus) of human   ZNF746.

Application Notes

Western blot was preformed on mouse   lung tissue using this antibody (Zhang et al, 2014; PMID:24778451). This   antibody was used for immunohistochemistry on mouse brains (Brahmachari et   al, 2019; PMID:31237944). The antibody was used in the immunopercipitation of   proteins from the brain of a mouse (Brahmachari et al, 2019; PMID:31237944).

Antibody First Published

Zhang et al. Endothelial PINK1   mediates the protective effects of NLRP3 deficiency during lethal oxidant   injury J Immunol. 2014 Jun 1;192(11):5296-304. PMID:24778451

Note on publication

This article describes research on the   protective effects of NLRP3 deficiency during lethal oxidant injury. And   describes the use of this antibody in western blotting lung tissue

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.