Key features and details | |
Cat. No. | MABL-153 |
Name | Anti-HCN1 mAbs |
Clone No. | AFD-N70/28 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ICC, immunogold labeling, WB, IF, IHC |
Species Reactivity | Rat, Human, Mouse |
Basic Information | |
Specificity | This antibody is specific for the amino acids 778-910 of the HCN1 protein. HCN1 is a hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). |
Alternative Name | N70/28R; Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1 |
UniProt | Q9JKB0 |
Immunogen | This antibody was raised by immunising BALB/c mice with a fusion of protein amino acids 778-910 (cytoplasmic C-terminus) of rat HCN1. |
Application Notes | This antibody was used in a immunoblotting experiment on mouse neural tissue (Arimitsu et al, 2009; PMID:19563776). Immunohistochemistry was preformed on forebrain tissue of mice using this antibody (Zhou et al, 2013; PMID:23377220). The ganglion tissue of CBA/CaJ mice was used in a immunocytochemistry experiment using this antibody (Liu et al, 2014; PMID:24558054). Immunogold labeling was preformed on coronal sections of the brain of mice using this antibody (Huang et al, 2017; PMID:28071723). This antibody was used in a immunoblotting experiment on rat retinal ganglion cells (Stradleigh et al, 2011; PMID:21456027). Immunofluorescence was preformed on the brain of mice using this antibody (Kole et al, 2015; PMID:26269648). |
Antibody First Published | Arimitsu et al. Activity-dependent regulation of HCN1 protein in cortical neurons Biochem Biophys Res Commun. 2009 Sep 11;387(1):87-91. PMID:19563776 |
Note on publication | Cortical cultured neurons and biochemical methods were used to identify molecular and cellular mechanisms that mediate the physiological regulation of HCN1 channel functions in cortical neurons. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
