Key features and details | |
Cat. No. | MABL-154 |
Name | Anti-Ataxin-1, 11NQ mAbs |
Clone No. | AFD-N76/3 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, IF, IHC |
Species Reactivity | Rat, Human, Mouse |
Basic Information | |
Specificity | This antibody is specific for amino acids 778-910 of HCN1. It shows no cross-reactivity against HCN2. HCN1 is a chromatin-binding factor that repress Notch signaling in the absence of Notch intracellular domain by acting as a CBF1 corepressor. |
Alternative Name | N76/3R; Atxn1; Spinocerebellar ataxia type 1 protein homolog; Ataxin-1 |
UniProt | P54254 |
Immunogen | This antibody was raised by immunising BALB/c mice with a fusion protein amino acids 778- 910 (cytoplasmic C-terminus) of rat HCN1. |
Application Notes | This antibody has been used in western blot on the brain of a mouse (Hu et al, 2019; PMID:30649233). Immunohistrochemistry on the brain of a mouse has been preformed with this antibody (Hu et al, 2019; PMID:30649233). The antibody has shown use in immunofluorescence experiments on neurospheres, differentiates cells and cerebral tissues of mice (Edamakanti et al, 2018; PMID:29533923). |
Antibody First Published | Edamakanti et al. Mutant ataxin1 disrupts cerebellar development in spinocerebellar ataxia type 1 J Clin Invest. 2018 Jun 1;128(6):2252-2265. PMID:29533923 |
Note on publication | Given the importance of this postnatal period for cerebellar development, it was asked whether this region might be developmentally altered by mutant ATXN1. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
