Anti-Ataxin-1, 11NQ mAbs

Cat. No.

MABL-155

Name

Anti-Ataxin-1, 11NQ  mAbs

Clone No.

AFD-N76/8

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IP, WB, IHC

Species Reactivity

Rat, Human, Mouse

Basic Information

Specificity

This antibody is specific for amino acids   164-197 of HCN1. It shows no cross-reactivity against HCN2. HCN1 is a   chromatin-binding factor that repress Notch signaling in the absence of Notch   intracellular domain by acting as a CBF1 corepressor.

Alternative Name

N76/8R; Atxn1; Spinocerebellar ataxia type 1   protein homolog; Ataxin-1

UniProt

P54254

Immunogen

This antibody was raised by immunising   BALB/c mice with a synthetic peptide amino acids 164-197 of mouse ataxin-1.

Application Notes

This antibody has been used in a   western blot experiment on mouse brain lysates and HeLa cell lysates (Suh et   al, 2019; PMID:31442405) (Barclay et al, 2014; PMID:24179173). Immunohistochemistry was preformed with this antibody on the brain of mice   (Suh et al, 2019; PMID:31442405). The antibody has shown use   immunoprecipitation experiments of HeLa cell lysate (Barclay et al, 2014;   PMID:24179173).

Antibody First Published

Barclay et al. Systems biology   analysis of Drosophila in vivo screen data elucidates core networks for DNA   damage repair in SCA1 Hum Mol Genet. 2014 Mar 1;23(5):1345-64. PMID:24179173

Note on publication

they performed a systematic in vivo   screen of all available Drosophila melanogaster homolog DNA repair genes, and   we tested the effect of their overexpression on lifespan and developmental viability in Spinocerebellar Ataxia Type 1 (SCA1) Drosophila models   expressing human mutant Ataxin-1 (Atxn1).

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.