Anti-GABA(B)R mAbs

Cat. No.

MABL-162

Name

Anti-GABA(B)R mAbs

Clone No.

AFD-N93A/49.2

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IP, PLA, IF

Species Reactivity

Rat, Human, Mouse

Basic Information

Specificity

This antibody is specific for amino acids 873-977   (cytoplasmic C-terminus) of rat GABABR1. GABABR1 is a component of a   heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and   GABBR2. Within the heterodimeric GABA receptor, only GABBR1 seems to bind   agonists, while GABBR2 mediates coupling to G proteins.

Alternative Name

N93A/49.2R; Gamma-aminobutyric acid type B   receptor subunit 1; Gabbr1; GABA-B receptor 1; GABA-B-R1; GABA-BR1; GABABR1; Gb1

UniProt

Q9Z0U4

Immunogen

This antibody was raised by immunising   BALB/c mice with a fusion protein of amino acids 873-977 (cytoplasmic   C-terminus) of rat GABABR1.

Application Notes

This antibody was used in a proximity   ligation assay on HEK293 cells transiently transfected using Lipofectamine   2000 with cDNA plasmids expressing GABABR1-eYFP, GABABR2 and GIRK2a (Li et   al, 2020; PMID:32111696). Immunofluorescence was preformed on the hippocampal   slices of P7 male Wistar rats using this antibody (Wright et al, 2017;   PMID:28450542). An immunoprecipitation was preformed with this antibody on   lysates from hippocampal slices of P7 male Wistar rats (Wright et al, 2017;   PMID:28450542). Immunofluorescence was preformed on the brain of   Tg(Gng3-GFP)- HK208Gsat and KI(Gng7-3-IRES-GFP) mice (Schwindinger et al,   2012; PMID:22207761).

Antibody First Published

Schwindinger et al. Synergistic   roles for G-protein γ3 and γ7 subtypes in seizure susceptibility as revealed   in double knock-out mice J Biol Chem. 2012 Mar 2;287(10):7121-33.PMID:22207761

Note on publication

A gene targeting approach to   determine whether the closely related γ(3) and γ(7) subunits can perform   functionally interchangeable roles in mice was used.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.