Anti-Neuroligin-1 mAbs

Cat. No.

MABL-165

Name

Anti-Neuroligin-1  mAbs

Clone No.

AFD-N97A/31

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ICC, WB, IF, IHC

Species Reactivity

Rat, Mouse

Basic Information

Specificity

This antibody is specific for amino   acids 718-843 (intracellular C-terminus) of rat Neuroligin-1. The antibody   does not cross-react with Neuroligin-2, -3, -4 or -4*. Neuroligin-1 is a cell   surface protein involved in cell-cell-interactions via its interactions with   neurexin family members. Plays a role in synapse function and synaptic signal   transmission, and probably mediates its effects by recruiting and clustering other synaptic proteins.

Alternative Name

N97A/31R; Nlgn1; Neuroligin I

UniProt

Q62765

Immunogen

This antibody was raised by immunising BALB/c mice with a fusion protein of amino acids 718-843   (intracellular C-terminus) of rat Neuroligin-1.

Application Notes

This antibody has been used for immunoblotting   on neuronal cultures were from embryonic day Wistar rat brains (Binda et al,   2019; PMID:30602588). Immunocytochemistry was preformed on mouse low density   hippocampal cultures using this antibody (Frei et al, 2020). An   immunostaining experiment was preformed on mouse neuronal cells using this   antibody (Yamagata et al, 2018; PMID:29760652). Mouse hippocampi were   dissected and immunoblotted using this antibody (Hoy et al, 2013;   PMID:23719805). Immunocytochemistry was preformed using cultured, E18-derived,   rat hippocampal neurons using this antibody (Kim et al, 2020; PMID:32434929).   This antibody was used in a immunohistochemistry experiment on mouse brain   sections (Kim et al, 2020; PMID:32434929).

Antibody First Published

Hoy et al. Neuroligin1 drives   synaptic and behavioral maturation through intracellular interactions J   Neurosci. 2013 May 29;33(22):9364-84. PMID:23719805

Note on publication

 The synaptic and behavioral phenotypes of   mice overexpressing a full-length version of NL1 (NL1FL) was comapred with   mice overexpressing a version missing part of the intracellular domain   (NL1ΔC).

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.