Key features and details | |
Cat. No. | MABL-220 |
Name | Anti-iC3b mAbs |
Clone No. | AFD-6G1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, ELISA, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody recognizes a conformational epitope on the human iC3b. |
Alternative Name | inactivated C3b; Complement C3; C3; Complement C3 beta chain |
UniProt | P01024 |
Immunogen | The original antibody was generated by immunizing mice intraperitoneally with human iC3b protein mixed with RIBI djuvant. |
Application Notes | This antibody binds iC3b with a dissociation constant of 43.3 nM. This antibody can be used in combination with 5D2 to detect iC3b in a sanwich ELISA. This antibody was used to identify iC3b using immunoprecipitation. This antbody was also used for the immunohistochemical analysis of Alzheimer's disease brain and was successful in labelling plaques with various morphologies in the grey matter at a 0.625 μg/mL dilution. This antibody can successfully stain human bruch's membrane/choroid tissues samples from patients having age related macular degeneration (AMD). It was able to identify drusen associated with bruch's membrane in stage 3 and 4 AMD tissues (US20140127225). |
Antibody First Published | US20140127225 |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
