Key features and details | |
Cat. No. | MABL-219 |
Name | Anti-Amyloid Beta mAbs |
Clone No. | AFD-21F12 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB, ELISA, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody recognizes amino acids 33-42 of amyloid beta. |
Alternative Name | Abeta; A-beta; Aβ; Aβ42; Alzheimer disease amyloid protein; S-APP-beta |
UniProt | P05067 |
Immunogen | The original antibody was generated by immunizing A/J mice with a synthetic peptide (NH2- CYS-NH-CH2-(CH2)5-CO-GLMVGGVVIA-COOH) as an immunogen. |
Application Notes | This antibody has very high specificity for Aβ1–42 over Aβ1–40. This antibody is capable of immunoprecipitating Aβ peptides ending at aa position 42 and also p3 peptides (PMID: 8917563). This antibody is also recomended for detection of amyloid beta using sandwich ELISA (PMID: 9037091, 19204155). This antibody was also used in the quantification of the proportion of full-length Aβ using western blotting (PMID: 12787077). This antibody has also been used for the identification of Aβ42 plaques on paraffin frontal sections(PMID: 15215183, 19660650). This antibody is unable to bind plaques in unfixed brain sections. This antibody is also not capable of clearing plaque after in vivo administration (PMID: 10932230). |
Antibody First Published | Citron et al. Evidence that the 42- and 40-amino acid forms of amyloid beta protein are generated from the beta-amyloid precursor protein by different protease activities. Proc Natl Acad Sci U S A. (1996); 93(23):13170-5. PMID:8917563 |
Note on publication | Describes the generation of different amino acid forms of amyloid beta by different protease activities. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
