Key features and details | |
Cat. No. | MABL-238 |
Name | Anti-Cn2 toxin mAbs |
Clone No. | AFD-BCF2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | neutralize, RIA, ELISA |
Species Reactivity | Centruroides noxius (scorpion) |
Basic Information | |
Specificity | This antibody binds the mammal-specific Cn2 toxin found in the venom produced by Centruroides noxius Hoffmann (Mexican scorpion). This antibody binds an epitope on the Cn2 toxin that is different from the binding epitope of another anti-Cn2 toxin antibody 6009F. |
Alternative Name | scorpion toxin; Beta-mammal toxin Cn2; Toxin 2; Toxin II.9.2.2; scorpion venom |
UniProt | P01495 |
Immunogen | The original antibody was generated by immunizing female BALB/c mice with a conjugate comprising thyroglobulin and a fraction II-9.2 (which contains toxins 2 and 3) of the Centruroides noxius (mexican scorpion) venom in Freund's complete adjuvant. |
Application Notes | The binding specificity and characterization of this antibody towards Cn2 toxin of scorpion Centruroicles noxius was tested using ELISA and RIA. This antibody was capable of inhibiting binding of C. noxius toxin 2 to rat brain membranes in vitro and neutralizing the toxin acitivity in vivo in mice (PMID: 1371253). It was reported that the Fab fragment of this antibody was capable of neutralizing both the Cn2 toxin and whole soluble venom of scorpion Centruroides noxius (PMID: 8875771). The scFv version of this atnbidoy did not shoe any neutralization acitivity in vivo due to its less stability (PMID: 15713481). |
Antibody First Published | Zamudio et al. Amino acid sequence and immunological characterization with monoclonal antibodies of two toxins from the venom of the scorpion Centruroides noxius Hoffmann. Eur J Biochem. 1992 Feb 15;204(1):281-92. PMID:1371253 |
Note on publication | Describes the characterization of the toxins from scorpion Centruroides noxius using antibodies. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
