Key features and details | |
Cat. No. | MABL-239 |
Name | Anti-Cn2 toxin mAbs |
Clone No. | AFD-6009F |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | neutralize, ELISA |
Species Reactivity | Centruroides noxius (scorpion) |
Basic Information | |
Specificity | This antibody binds the mammal-specific Cn2 toxin found in the venom produced by Centruroides noxius Hoffmann (Mexican scorpion). This antibody binds an epitope on the Cn2 toxin that is different from the binding epitope of another anti-Cn2 toxin antibody BCF2. It was also reported that this antibody cross-reacts with other toxins like Cn3, Css2, and Css4. Cross-reactivity against Css2 was relevant, because this toxin is the major constituent of the venom of Centruroides suffusus (PMID: 21156801). |
Alternative Name | scorpion toxin; Beta-mammal toxin Cn2; Toxin 2; Toxin II.9.2.2; scorpion venom |
UniProt | P01495 |
Immunogen | The original antibody was isolated from a human non-immune phage display library by panning the library against Cn2 toxin of scorpion Centruroides noxius. |
Application Notes | The binding specificity and characterization of this antibody towards Cn2 toxin of scorpion Centruroicles noxius was tested using ELISA. The epitope mapping of this antibody was performed using competitive ELISA. The scFv version of this antibody binds Cn2 toxin with an affinity of 410pM and capable of neutralizing 2 LD50 of Cn2 toxin when a 1 : 10 molar ratio of toxin-to-antibody fragment was used. It was also able to neutralize 2 LD50 of the whole venom (PMID: 15885107; US7381802). ELISA was also used to determine the cross- reactivity of this antibody towards other toxins like Cn3, Css2, and Css4 (PMID: 21156801). |
Antibody First Published | Riaño-Umbarila et al. A strategy for the generation of specific human antibodies by directed evolution and phage display. An example of a single-chain antibody fragment that neutralizes a major component of scorpion venom. FEBS J. 2005 May;272(10):2591-601. PMID:15885107 |
Note on publication | Describes the generation of this antibody using phage display technique and also describes the affinity maturation of this antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
