Key features and details | |
Cat. No. | MABL-246 |
Name | Anti-Methamphetamine mAbs |
Clone No. | AFD-6H4 S93T |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | RIA, therapeutic, ELISA |
Species Reactivity | Species independent |
Basic Information | |
Specificity | This antibody is capable of recognizing methamphetamine, amphetamine, 3,4- methylenedioxymethamphetamine (MDMA) and 4-OH-METH. Methamphetamine is a potent central nervous system (CNS) stimulant that is mainly used as a recreational drug and less commonly as a second-line treatment for attention deficit hyperactivity disorder and obesity. In low to moderate doses, methamphetamine can elevate mood, increase alertness, concentration and energy in fatigued individuals, reduce appetite, and promote weight loss. At very high doses, it can induce psychosis, breakdown of skeletal muscle, seizures and bleeding in the brain. Chronic high-dose use can precipitate unpredictable and rapid mood swings, stimulant psychosis (e.g., paranoia, hallucinations, delirium, and delusions) and violent behavior. |
Alternative Name | METH; Ecstasy; MDMA; AMP; N-methylamphetamine; amphetamine; N α- dimethylphenethylamine; desoxyephedrine; crystal meth; drug; CAS:537-46-2; CHEBI:6809; ChEMBL:1201201; (+)-4-(5-carboxypentyl)methamphetamine; (+)3 4-methylenedioxymethamphetamine; 4- OH-METH |
UniProt | |
Immunogen | The original antibody was generated by immunizing female BALB/c mice with a hapten called METH P6 or (S)-(+)-4-(5-carboxypentyl)methamphetamine. Later on a point mutation was introduced into the original antibody to generate an affinity improved version. |
Application Notes | This antibody is an affinity improved version of antibody 6H4 that has a 3.1 fold enhancement in affinity for METH and 26 fold for AMP (PMID: 24419156). The original IgG1 version of 6H4 antibody binds methamphetamine with a binding affinity of Kd=11nM. The initial binding characterization of this antibody was done using ELISA (US7858756). The scFv version of this antibody was reported to bind (+)-METH with a binding affinity of kd=10nM. The binding affinity of the scFv version 6H4 for METH and similar drugs was determined by bead-based radioimmunoassay (RIA) (PMID: 18192498). To test the in vivo efficacy of the scFv6H4, male Sprague Dawley rats were implanted with 3-day sc osmotic pumps delivering 3.2 mg/kg/day METH. The scFv was stable for an extended period of time in vivo and was able to significantly redistribute METH into serum for at least 4 hrs. |
Antibody First Published | Thakkar et al. Affinity improvement of a therapeutic antibody to methamphetamine and amphetamine through structure-based antibody engineering. Sci Rep. 2014 Jan 14;4:3673. PMID:24419156 |
Note on publication | Describes the affinity improvement of an anti-methamphetamine antibody 6H4. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
