Key features and details | |
Cat. No. | MABL-247 |
Name | Anti-Prion protein mAbs |
Clone No. | AFD-D18 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | in vivo, inhibition, IP, SPR, WB, ELISA, FC |
Species Reactivity | Bovine, Syrian Hamster, Human, Mouse |
Basic Information | |
Specificity | This antibody recognises a discontinuous epitope and binds to residues 132-156 of PrP. |
Alternative Name | PrPC; ASCR; PrP27-30; PrP33-35C; CD230 |
UniProt | P04156 |
Immunogen | PrP deficient (Prnp0/0) mice were immunized with disaggregated PrP 27-30 incorporated into liposomes. The original antibody was generated by panning by panning against dispersed SHaPrP 27-30. |
Application Notes | The specificity of the antibody to recombinant SHaPrP(90-231) and with PrP 27-30 rods was confirmed by ELISA. The epitope mapping of this antibody was done using a paptide-based ELISA. The Fab fragment reacted with cell surface MoPrPc as showed by flow cytometry. The antibody efficiently immunoprecipitated SHaPrPC from transfected CHO cells (Williamson et al. 1998; PMID: 9765500). The ability of of the Fab fragment to inhibit prion propagation in a cultured mouse neuroblastoma cell line infected with PrPSc (ScN2a) was tested. The Fab fragment was found to abolish prion replication and to clear pre-exiting PrPSc, eliminating 50% of PrPSc from the cells within about 24 hours. Therefore, the antibody showed potential as a therapeutic agent. |
Antibody First Published | Williamson et al. Mapping the Prion Protein Using Recombinant Antibodies. J Virol. 1998 Nov; 72(11): 9413–9418. PMID:9765500 |
Note on publication | The paper describes the generation of a panel of monoclonal antibodies, which are used as probes for protein conformational study. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
