Key features and details | |
Cat. No. | MABL-250 |
Name | Anti-Phencyclidine mAbs |
Clone No. | AFD-6B5 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | antagonist, immunotherapy, RIA, ELISA |
Species Reactivity | Species independent |
Basic Information | |
Specificity | This antibody binds phencyclidine (PCP/ angel dust) and also has binding affinity for other aryl cyclohexylamines like tenocyclidine (TCP) and PCE. PCP is a dissociative hallucinogenic drug used for its mind-altering effects. PCP may cause hallucinations, distorted perceptions of sounds, and violent behavior. PCP can produce a dose-dependent psychosis that resembles schizophrenia with behavior described as extremely agitated, bizarre, unpredictable and paranoid. It is a dopamine receptor agonist and an antagonist at the N-methyl-d-aspartate (NMDA) subtype of glutamate receptors. It has anticholinergic properties through blockade of ion channels in acetylcholine receptors. It has been used in some countries as an anti-parkinsonian agent. |
Alternative Name | PCP; CI-395; Angel dust; PCE; TCP; phenyl cyclohexyl piperidine; aryl cyclohexylamine; tenocyclidine; 1-(1-phenylcyclohexyl)piperidine; CAS: 77-10-1; CHEBI:8058; mAb6B5; ch6B5; ch-mAb6B5 |
UniProt | |
Immunogen | The original antibody was generated from a mouse immunized with a phencyclidine analogue hapten 5-[N-(1′phenylcyclohexyl)amino]pentanoic acid (PCHAP) conjugated to bovine serum albumin. |
Application Notes | The binding characterization of this antibody to 3[H]PCP was done using radioimmunoassay. The original IgG1 version of this antibody binds phencyclidine with an affinity of Kd=1.3 nM. (PMID: 9618414). This antibody was useful in reversing PCP-induced central nervous system toxicity in laboratory animals. The Fab version of this antibody induced antagonism of behavioral effects of repeated intravenous PCP challenges. The Fab completely reversed all PCP-induced locomotor effects in a dose dependent manner. It also completely reversed the locomotor effects induced by two other structurally related potent analogs of PCP: 1-[1-(2-thienyl)cyclohexyl]piperidine and N-ethyl-1- phenylcyclohexylamine (PMID: 9618414). In a rat model for human acute PCP overdose, the antigen- binding fragment (Fab) of mAb6B5 causes a rapid and effective redistribution of PCP out of the brain (PMID:8768723). |
Antibody First Published | Hardin et al. Pharmacodynamics of a monoclonal antiphencyclidine Fab with broad selectivity for phencyclidine-like drugs. J Pharmacol Exp Ther. 1998 Jun;285(3):1113-22. PMID:9618414 |
Note on publication | Describes the generation of the Fab fragment and tests its usefulness in the treatment of drug intoxication. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
