Anti-14G2a mAbs

Cat. No.

MABL-287

Name

Anti-14G2a mAbs

Clone No.

AFD-4B5

From

Recombinant Antibody

Isotype

Engineer antibody

Application

funtional assay, RIA, ELISA

Species Reactivity

Mouse

Basic Information

Specificity

This antibody is specific for 14G2a. 14G2a is a mouse IgG2a antibody specific for human GD2.

Alternative Name

antibody 14G2a; ganglioside; ganglioside 2a

UniProt

Immunogen

The original human version of this  antibody was generated by treating humans with the 14G2a antibody then MNCs   were harvested and hybridized with the murine myeloma cell line P3X.Ag8.653.

Application Notes

To determine the functionality of   the mouse version of this antibody, an ELISA was performed to test if this   antibody was able to bind to antigen 14G2A. Furthermore, the antibody was   also tested in a western blot on antigen 14G2A (EP1000082A2). A radioimmunoassay  was performed to screen for anti-14G2a activity of the human version of this   antibody. To assess the ability of the human version of this antibody to   mediate a cellular immune response. Rabbits were immunized with 4B5. Then,   irradiated Mel-21 cells were implanted. The result was induration at the site   of the irradiated cells (Saleh et al., 1993; PMID: 8376782).

Antibody First Published

Saleh et al. Generation of a human   anti-idiotypic antibody that mimics the GD2 antigen J Immunol. 1993 Sep   15;151(6):3390-8. PMID:837678

Note on publication

Four human anti-14G2a secreting   hybridomas were generated and the mAb product of one of the hybridomas was   characterized.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.