Key features and details | |
Cat. No. | MABL-3179 |
Name | Anti-VACV mAbs |
Clone No. | AFD- SC212 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | in vitro neutralization, ELISA |
Species Reactivity | Vaccinia virus |
Basic Information | |
Specificity | The antibody is specific for VACV. |
Alternative Name | VV |
UniProt | |
Immunogen | A phage display library of human single chain variable fragment (scFv) antibodies was constructed from the B cells of healthy volunteers boosted by the VACV vaccine. Human scFv was isolated by panning against VACV. |
Application Notes | The scFv fragment was converted to human IgG1 (VC212). The binding affinity of VC212 was estimated by competition ELISA to IC50 values 22 µg/ml (146.67 nM). The humanized version of the antibody neutralized the VACV, as evidenced by the plaque reduction neutralization test result with a PRNT50 of ~0.16 mg/ml (~1.07 µM) (Shin et al., 2019; PMID: 30856707). |
Antibody First Published | Shin et al. Selection of Vaccinia Virus-Neutralizing Antibody from a Phage- Display Human-Antibody Library J Microbiol Biotechnol. 2019 Apr 28;29(4):651-657. PMID:30856707 |
Note on publication | The original paper describes the generation and characterization of the antibody |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
