Anti-CD105 mAbs

Cat. No.

MABL-670

Name

Anti-CD105 mAbs

Clone No.

AFD- huRH105-1

From

Recombinant Antibody

Isotype

Engineer antibody

Application

in vitro, in vivo, FC

Species Reactivity

Rat, Human

Basic Information

Specificity

The antibody binds to human and rat   endoglin.

Alternative Name

endoglin; ENG

UniProt

P17813

Immunogen

The original mouse parental antibody RH105-1   was generated by intraperitoneal immunization of BALB/c VAF mice with rat   CD105-expressing 300-19 cells. This antibody was synthetically engineered to   mimic a binding specificity of the original RH105-1, by grafting the CDRs of   the parental antibody onto human framework segments.

Application Notes

This antibody was used for detection of   CD105 expressed on Vero cell lines by flow cytometry (Kd = 0.024 nM). The   ability of the antibody to induce apoptosis on HUVEC cells was measured.   Results showed that treatment with the antibody induced apoptosis of up to   47% of HUVEC cells at a concentration of 0.25 μg/mL. Further, the antibody   induced a greater percentage of cells to undergo apoptosis under hypoxic   conditions. The binding affinity of the antibody and the antibody conjugated   to SPDB-DM4 was assayed by flow cytometry. The value of the apparent   dissociation constants were calculated Kd = 1.63 nM and 2.12 nM,   respectively. Treatment of HUVEC cells with huRH105-l-SMCC-DMl completely   reduced viability of HUVEC cells with an EC50 of 0.12 nM in in vitro cytotoxicity   experiments, while treatment of HUVEC cells with huRH105-l-SPDB-DM4   completely reduced viability with an EC50 of 0.21 nM. 

Antibody First Published

Note on publication

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.