Anti-CD104 mAbs

Cat. No.

MABL-669

Name

Anti-CD104 mAbs

Clone No.

AFD- 58XB4

From

Recombinant Antibody

Isotype

Engineer antibody

Application

FC, IHC

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific for human CD104. Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in   the hemidesmosome of epithelial cells. Is required for the regulation of   keratinocyte polarity and motility.

Alternative Name

Integrin beta-4; ITGB4; GP150

UniProt

P16144

Immunogen

The mouse version of this antibody was   raised by immunizing a BALB/c mouse with the full length CD104 protein.

Application Notes

To investigate the recruitment of plectin   into hemidesmosomes by the α6β4 integrin, immunohistochemistry was performed   on human MD-EBS keratinocytes using the mouse version of this antibody   (Geerts et al, 1999; pmid:10525545). While showing that a subpopulation of   cells in a hiPSC- derived corneal epithelial cell sheet is positive for   ABCB5, flow cytometry was performed on human hiPSC- derived SEAMs using the   mouse version of this antibody (Watanabe et al, 2021; pmid:34195566). The   mouse version of this antibody was used in flow cytometry on differentiated   human iPS cells to demonstrate the generation of a self-formed ectodermal   autonomous multi-zone (SEAM) of ocular cells using human induced pluripotent   stem cells (Hayashi et al, 2016; pmid:26958835).

Antibody First Published

Geerts et al. Binding of integrin   alpha6beta4 to plectin prevents plectin association with F-actin but does not   interfere with intermediate filament binding J Cell Biol. 1999 Oct   18;147(2):417-34. PMID:10525545

Note on publication

The recruitment of plectin into   hemidesmosomes by the alpha6beta4 integrin was investigated and it has been   shown that the cytoplasmic domain of the beta4 subunit associates with an   NH(2)-terminal fragment of plectin that contains the actin-binding domain   (ABD).

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.