Key features and details | |
Cat. No. | MABL-681 |
Name | Anti-CD11a mAbs |
Clone No. | AFD- AL-57 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallization, SPR, ELISA, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody binds to the alpha L I domain in a HA but not LA conformation.The antibody binding site overlaps the ICAM-1 binding site on the I domain. |
Alternative Name | LFA-1A; integrin LFA-1; Integrin alpha-L; activated LFA-1 clone 57; CD11 antigen-like family member A; Leukocyte adhesion glycoprotein LFA-1 alpha chain ;Leukocyte function- associated molecule 1 alpha chain |
UniProt | P20701 |
Immunogen | The original antibody was selected from a human Fab-displaying library against the HA open conformation of the LFA-1 I domain. |
Application Notes | The specificity of the original format of the antibody was confirmed by ELISA analysis. The cell-binding specificity of the original and IgG1 format of the antibody was determined using flow cytometric analysis with HA and LA cells, the antibody bound to HA cells in a dose-dependent manner but did not bind to LA cells. The antibody preferentially binds to the active conformation of LFA-1 and blocks LFA-1-mediated adhesion and lymphocyte proliferation (Huang et al., 2006; PMID: 16888085). The original format of the antibody could discriminate among low-affinity, intermediate-affinity (IA), and HA states of LFA-1. Furthermore, the binding of the antibody depended on the presence of Mg2+. The antibody showed no binding to the WT I domain, intermediate binding to the IA I domain (KD = 4.7 μM), and good binding to the HA I domain by SPR (KD = 0.023 μM). |
Antibody First Published | Huang et al. Identification and characterization of a human monoclonal antagonistic antibody AL-57 that preferentially binds the high-affinity form of lymphocyte function- associated antigen- J Leukoc Biol. 2006 Oct;80(4):905-14. doi: 10.1189/jlb.1105649. Epub 2006 Aug 3. PMID:16888085 |
Note on publication | The original paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
