Key features and details | |
Cat. No. | MABL-682 |
Name | Anti-CD11a mAbs |
Clone No. | AFD- MHM24 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | cytofluorography, humanisation, inhibition studies |
Species Reactivity | Human |
Basic Information | |
Specificity | MHM24 binds to the CD11a subunit of lymphocyte function-associated antigen. It binds to both the high- and low-affinity forms of the antigen. |
Alternative Name | Integrin alpha-L; CD11 antigen-like family member A; leukocyte function- associated molecule 1 alpha chain; human lymphocyte function antigen |
UniProt | P20701 |
Immunogen | Spleen cells from BALB/c mice immunised with EBV-transformed human lymphoblastoid cells were fused with P3-NSUlAg4.1 myeloma cells and the resulting hybridomas were screened for antibody production by a radioimmunoassay on T and B cell lines. |
Application Notes | Cytofluorography was used to show the binding of MHM24 to peripheral blood lymphocytes, with 97.3% of cells stained by MHM24 (Hildreth et al, 1983). In inhibition experiments, MHM24 inhibited T cell-mediated lysis of virus-infected cells (Hildreth et al, 1983). Additionally, MHM24 inhibited NK cell-mediated lysis (Hildreth et al, 1983).Flow cytometry showed MHM24 exhibits non-selective binding to high-affinity and low-affinity forms of LFA-1 (Huang et al, 2006). Murine MHM24 was humanised and shown to prevent adhesion of human T cells to human keratinocytes and the proliferation of T cells in response to nonautologous leukocytes (Werther et al, 1996). |
Antibody First Published | Hildreth et al, 1983. A human lymphocyte-associated antigen involved in cell-mediated lympholysis. Eur J Immunol. 1983 Mar;13(3):202-8. PMID:6339253 |
Note on publication | Describes the original generation of the antibody and its characterisation through inhibition studies. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
