Key features and details | |
Cat. No. | MABL-689 |
Name | Anti-CD11b mAbs |
Clone No. | AFD- ICRF44 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB, FC, IHC |
Species Reactivity | chimpanzee, Pig, Human, Rhesus Monkey, Cynomolgus Monkey |
Basic Information | |
Specificity | This antibody recognizes CD11b (Integrin alpha-M) which is an adhesion molecule. CD11b associated with integrin beta2 (CD18) is expressed on the surface of monocytes, granulocytes, activated lymphocytes and a subset of NK cells. CD11b is a receptor for intercellular adhesion molecule family members CD54, CD102 and CD50 as well as for iC3b. These adhesions are crucial in cell-cell and cell-matrix interactions. |
Alternative Name | Integrin alpha-M; CD11 antigen-like family member B; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Leukocyte adhesion receptor MO1; Neutrophil adherence receptor; mAb 44; mAb44 |
UniProt | P11215 |
Immunogen | The original mouse IgG1 version of this antibody was raised against the rheumatoid synovial cells and human monocytes. |
Application Notes | This antibody is recommended for the detection and analysis of CD11b (Integrin alpha- M) and CD11b-plus cells in lineage identification. The original mouse IgG1 format of this clone has been widely used in various flow cytometry experiments. For instance, ICRF44 was applied in an FC analysis of cells during MDSCs' generation (Eriksson et al., 2019; pmid: 30617223). Another group screened mouce splenocytes with this antibody during tumor studies (Horn et al., 2017; pmid: 28938530). Jardine et al. (2019) utilized this clone to phenotype blood DC cells (pmid: 31040289). Similarly, this antibody was used as a part of a wider panel to phenotype MUTZ-3 and MUTZ-LCs cells (Bock et al., 2016; pmid: 26794428). |
Antibody First Published | Malhotra et al. Ligand binding by the p150,95 antigen of U937 monocytic cells properties in common with complement receptor type 3 (CR3) Eur J Immunol. 1986 Sep;16(9):1117-23. doi: 10.1002/eji.1830160915. PMID:3530784 |
Note on publication | This article describes the characterization of the ICRF44 antibody (mAb 44). |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
