Anti-CD11c mAbs

Cat. No.

MABL-693

Name

Anti-CD11c mAbs

Clone No.

AFD- N418

From

Recombinant Antibody

Isotype

Engineer antibody

Application

FACS, in vivo, FC, IF

Species Reactivity

Human, Mouse

Basic Information

Specificity

The antibody binds to mouse and human CD11c.

Alternative Name

Integrin alpha-X

UniProt

P20702

Immunogen

Hamster spleen cells that had been primed to   mouse spleen dendritic cells

Application Notes

This antibody binds to human CD11, which is part of the human integrin receptor for fibrinogen, recognizing a GPR motif. The receptor is involved in cell-cell contacts, chemotaxis, and inflammatory   responses. The binding of this antibody to its target was originally   evaluated via FACS (Metlay et al., 1990; PMID: 2185332). This antibody was   used for FC (Liu et al., 1998; PMID: 9723687). This antibody was used in vivo   as a tool to target the delivery of a model antigen specifically to dendritic   cells in mice, resulting in rapid and high antibody response (Wang et al.,   2000; PMID: 10639168). Conjugating this antibody to synthetic peptides   enhanced their immunogenicity (Berry et al., 2003; PMID: 10639168). This   antibody was successfully used in an IF assay (AbAb internal results).

Antibody First Published

Metlay et al. The distinct leukocyte   integrins of mouse spleen dendritic cells as identified with new hamster   monoclonal antibodies. Journal of Experimental Medicine 1990; 171(5):1753- 71   PMID:2185332

Note on publication

Describes the generation of several hamster   monoclonal antibodies against human dendritic cells.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.