Key features and details | |
Cat. No. | MABL-697 |
Name | Anti-CD127 mAbs |
Clone No. | AFD- GR34 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | antagonist, Block, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds human IL-7R. |
Alternative Name | IL7R; IL7Rα; IL-7Rα; Interleukin-7 receptor subunit alpha; IL-7 receptor subunit alpha; IL-7R-alpha; IL-7RA; CDw127 |
UniProt | P16871 |
Immunogen | The original parental clone was generated by immunizing BALB/c mice with Pre-Alp, a t(l;19) pre-B leukemic cell line. |
Application Notes | This IL-7R antagonist antibody is a recombinant engineered version of clone R34.34. The binding affinity of this antibody for human IL-7R was checked using ELISA. Antibody GR34 (mouse IgG1 version) was determined to inhibit pSTAT5 signalling with an IC50of 0.22μg/ml. The mouse IgG1 version had an affinity for the IL-7Rα EDC of 15.3nM (KD) and a Kd of 8.75X10-4. The mouse IgG1 version was capable of binding to IL-7Rα expressed on CHO cells with an EC50 of 0.27μg/ml, and blocked IL-7/IL-7Rα with an IC50of 2.29μg/ml (WO2010017468). |
Antibody First Published | Leung et al. Treatment of autoimmune and inflammatory disease. WO2010017468 |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
