Key features and details | |
Cat. No. | MABL-699 |
Name | Anti-CD13 mAbs |
Clone No. | AFD- WM15 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | in vitro, FC, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for human CD13, but its epitope is destroyed during the process of formalin fixation and paraffin embedding. |
Alternative Name | gp150; AP-M; AP-N; hAPN; ANPEP; APN; PEPN; Alanyl aminopeptidase; Aminopeptidase N; Aminopeptidase M; Microsomal aminopeptidase; Myeloid plasma membrane glycoprotein CD13 |
UniProt | P15144 |
Immunogen | The orignal antibody was raised by immunizing a mouse with human CD20. |
Application Notes | The original antibod was used for immunohistochemsitry on a range of human inflammatory tissue specimens. This antibody was used for flow cytometry on CD31+ CD45- endothelial cells identified with mouse anti-human FITC-CD31 (Di Matteo et al., 2011; PMID:21339174), and on he human monocytoid cell line U937 (Löhn et al., 2002; PMID:11999577). This antibody was used for an in vitro wound scratch assay on human fibroblasts, it showed a 42% decrease in cell migratory activity. This antibody was used for a proliferation assay on human fibroblasts, it showed that the antibody did not affect the proliferation of fibroblasts (Lai et al., 2010; PMID:20589526). |
Antibody First Published | Löhn et al. Cell cycle retardation in monocytoid cells induced by aminopeptidase N (CD13) Leuk Lymphoma. 2002 Feb;43(2):407-13. PMID:11999577 |
Note on publication | In the original paper the physiologiucal role of CD13 expression on human leucocytes was investigated. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
