Key features and details | |
Cat. No. | MABL-702 |
Name | Anti-CD133 mAbs |
Clone No. | AFD-2C3 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody is specific for CD133. CD133 is a cell surface glycoprotein molecule mainly localized to the bulge of the cytoplasmic membrane and is involved in the organization of the membrane. CD133 is often used as a marker for tumor stem cell isolation and identification |
Alternative Name | Prominin-1; Antigen AC133; Prominin-like protein 1 |
UniProt | O43490 |
Immunogen | The original antibody was generated by screening a natural nanobody phage display library and panning against human CD133. |
Application Notes | The specificity of the antibody was confirmed by ELISA analysis. This antibody was used for detection of CD133 expressed on CHO cells by flow cytometry. The antibody human IgG1 Fc fusion protein was constructed and used for detection of CD133 expressed on HT29, HCT116 and Caco-2 cells. The antibody drug conjugate obtained by coupling 2C3-hFc with DM1, showed cytotoxic activity on HT29, HCT116 and Caco-2 cells (CN113480651B). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
