Key features and details | |
Cat. No. | MABL-703 |
Name | Anti-CD133 mAbs |
Clone No. | AFD- CD133-scFv-1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, ELISA, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody recognises CD133 which is thought to be engaged in cell differentiation, proliferation and apoptosis. |
Alternative Name | Prominin-1; AC133; Prominin-like protein 1 |
UniProt | O43490 |
Immunogen | This antibody fragment was selected against recombinant CD133 via phage display from the Tomlinson I and J libraries. |
Application Notes | CD133 is used as a cancer stem cell (CSCs) marker in a variety of carcinomas. High affinity of the CD133-scFv-1, which detects CD133, was confirmed via competitive ELISA agianst a commercailly available antibody directed against the same epitope (Xia et al., 2013). Immunoblot and flow cytometry were performed to demonstrate the specificity of the CD133-scFv-1 antibody in PLC/PRF/5 cells (Xia et al., 2013). |
Antibody First Published | Xia et al. Isolation, identification and expression of specific human CD133 antibodies. Sci Rep. 2013 Nov 25;3:3320. doi: 10.1038/srep03320. PMID:24271022 |
Note on publication | This article describes the generation and characterisation of the CD133-scFv-1 antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
