Key features and details | |
Cat. No. | MABL-705 |
Name | Anti-CD134 mAbs |
Clone No. | AFD- A10-F |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELIS, Block, FC |
Species Reactivity | Human, Rhesus Monkey, Cynomolgus Monkey |
Basic Information | |
Specificity | This antibody binds the human OX40, blocks binding of OX40L to OX40 and inhibits one or more functions associated with binding of OX40L to OX40. It also cross reacts with OX40 from cynomolgus and rhesus monkey. |
Alternative Name | OX40; OX-40; OX40L receptor; TNFRSF4; Tumor necrosis factor receptor superfamily member 4; ACT35 antigen; TAX transcriptionally-activated glycoprotein 1 receptor |
UniProt | P43489 |
Immunogen | The original antibody was generated by immunizing mice with a soluble OX40/Fcγ1 as an immunogen, combined with Freund's adjuvant (1:1) and administered to two six-week-old A/J mice. |
Application Notes | This antagonist antibody is a humanized version of the original murine parent antibody A10. The binding specificity of this antibody to OX40 was determined by ELISA and flow cytometric techniques. Naïve CD4+ T-cell assay was used to test the ability to inhibit cytokine production, proliferation, and to induce apoptosis in human CD4+ T-cells. A10-F inhibited cytokine release (IL-2/IL-5/IL- 13) and CD4+ T-cells proliferation to the same level as its murine parent. A10-F was also tested for its antagonistic activities like inhibiting the proliferation and cytokine release of Th1 primed T-cells, Th2 primed T-cells and Th17 cells in the presence of irradiated OX40L transfected L-cells or parental L-cells. |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
