Key features and details | |
Cat. No. | MABL-707 |
Name | Anti-CD134 mAbs |
Clone No. | AFD- OX86 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | agonist, ELISA, FC, IF |
Species Reactivity | Mouse |
Basic Information | |
Specificity | This antibody recognises murine OX40 (CD134), a cell surface antigen expressed only by activated T lymphocytes. It is a member of TNFR-superfamily of receptors and acts as a costimulatory molecule involved in long-term T-cell immunity. |
Alternative Name | OX40; OX-40; Tumor necrosis factor receptor superfamily member 4; OX40 antigen; OX40L receptor; CD134; Tnfrsf4; OX-86 |
UniProt | P47741 |
Immunogen | This antibody was raised against the recombinant mouse OX40 protein. |
Application Notes | OX86 antibody, in its original rat IgG1 format, acts as an OX40 agonist in an Fc- dependent manner and has been widely used in studies investigating the role of OX40 in tumor inhibition (Hirschhorn-Cymerman et al., 2009). Studies comparing two recombinant murine Fc versions (IgG1 and IgG2a) elucidated the importance of the FcR engagement in the antitumor activity of this antibody; the study demonstrated that a stronger murine Fc (in an IgG2a version) was more effective in inducing a CT26 syngeneic tumor regression as well as it led to a stronger Treg depletion in the tumor in comparison with a murine IgG1 version (Metzger et al., 2016). In another study two versions of the OX86 antibody (with a fully functional Fc fragment and with an impaired Fc) and showed that Fc-dependent activity was necessary in inducing systemic antitumor activity (Sagiv-Barfi et al., 2018). |
Antibody First Published | al-Shamkhani A, Birkeland ML, Puklavec M, Brown MH, James W, Barclay AN. OX40 is differentially expressed on activated rat and mouse T cells and is the sole receptor for the OX40 ligand. Eur J Immunol. 1996 Aug;26(8):1695-9.PMID:8765008 |
Note on publication | Describes the generation of the antibody, its use in flow cytometry (staining mouse CD4 and CD8 single-positive cells). |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
