Key features and details | |
Cat. No. | MABL-711 |
Name | Anti-CD138 mAbs |
Clone No. | AFD- OC-46F2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, Block, ELISA, FC, IF, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for the extracellular domain of human syndecan-1. Syndecan-1 is a cell membrane protein that, after its shedding by heparanase enzymes, is accumulated in the extracellular matrix of some tumours, e.g. myeloma and lung carcinoma, where it modulates several key processes of tumourigenesis such as cancer cell proliferation and apoptosis, angiogenesis and metastasis. |
Alternative Name | Syndecan-1; SDC1; SYND1; SDC |
UniProt | P18827 |
Immunogen | The original antibody was generated by selecting a human antibody phage display library from human melanoma cells and later on its by its expression in mammalian cells.. |
Application Notes | The original antibody inhibited vascular maturation and tumour growth in an experimental human melanoma model. The therapeutic efficacy of this antibody was also demonstrated in an experimental ovarian carcinoma model. OC-46F2 scFv version was used as a capture antibody in a solid phase sandwich ELISA (PMID: 26460958). The scFv version of this antibody was also used in the immunofluorescent and immunohistochemical analysis of melanoma tissues, human ovarian carcinomas, human normal ovary cells and SKMEL28 cells (PMID: 26460958, 26460958). Syndecan-1 expression on a panel of cell lines was analysed by flow cytometry using recombinant OC-46F2. The scFv antibody can also recognize purified human recombinant extracellular portion of syndecan-1 in a western blot assay. |
Antibody First Published | Orecchia et al. A novel human anti-syndecan-1 antibody inhibits vascular maturation and tumour growth in melanoma. Eur J Cancer. 2013 May;49(8):2022-33. PMID:23352437 |
Note on publication | Describes the generation of this antibody and tests its therapeutic efficacy in an experimental ovarian carcinoma model. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
