Key features and details | |
Cat. No. | MABL-716 |
Name | Anti-CD14 mAbs |
Clone No. | AFD- UCH-M1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, IF, IHC |
Species Reactivity | Pig, Human, Rhesus Monkey, Cynomolgus Monkey |
Basic Information | |
Specificity | This antibody is specific for human CD14, which is expressed strongly by macrophages and most tissue monocytes. It cross-reacts with non-human primate and pig CD14. |
Alternative Name | UCHM1; Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein |
UniProt | P08571 |
Immunogen | Human thymocytes followed by peripheral blood mononuclear cells. |
Application Notes | This antibody has been used to specifically stain monocytes and macrophages in cryostat sections of large cell lymphoma using an indirect immunoperoxidase technique (Addis et al, 1986) and cryostat lymph node sections (Salisbury et al, 1985). It has also been used to stain acetone-fixed cytocentrifuge preparations of a cell line derived from a patient with Hodgkin's disease (Jones et al, 1985). This antibody has been employed in an immunofluorescence analysis to assess CD14 expression in porcine epithelial cells (Liu et al, 2001), and in indirect immunofluorescence staining of cryostat thymus sections (Marinova et al, 2008). This antibody has also been used in Western blotting, and to deplete soluble CD14 from human sera through an immunoadsorption method (Shimizu et al, 2004). |
Antibody First Published | Jones et al. Phenotypic analysis of an established cell line derived from a patient with Hodgkin's disease (HD) Hematol Oncol. 1985 Apr-Jun;3(2):133-45. PMID:4018743 |
Note on publication | Describes the use of this antibody in IHC analysis |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
