Anti-CD142 mAbs

Cat. No.

MABL-717

Name

Anti-CD142 mAbs

Clone No.

AFD- 10H10 (TF9-10H10)

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ELISA, IF, IHC

Species Reactivity

Human

Basic Information

Specificity

This antibody binds the human tissue factor heavy chain protein (huTFh) and specifically binds huTFh polypeptide p1-30.

Alternative Name

TF; F3; Tissue factor; Coagulation factor   III; Thromboplastin

UniProt

P13726

Immunogen

The original antibody was generated by   subcutaneously immunizing a mouse with denatured human TF protein in R-700 adjuvant.

Application Notes

Tissue factor (TF), one of the cell-surface   initiators of blood coagulation and has been identified as a critical   controlling molecule in hemostasis, thrombosis and inflammation. This   antibody is recommended for detection of tissue factor in a blood sample.   This is a non neutralizing antibody, which is capable of binding human tissue   factor heavy chain protein but does not neutralize the ability of tissue   factor to initiate coagulation. This antibody was used to study the   interaction between factor VII and tissue factor in solution using ELISA (PMID: 1874730). This antibody is also reported to be used to target TF   pathway as a possible treatment strategy for glioblastoma therapy (PMID: 23384223). This antibody was used to study expression of TF in human glioma   using immunofluorescence staining for identification of TF in U251 glioma   cell line, normal brain specimens, and glioma surgical specimens (PMID: 12135696). This antibody was also used in the detection of TF in normal and   abnormal human mammary glands using immunohistochemistry (PMID: 1514249).

Antibody First Published

Morrissey et al. Monoclonal antibody   analysis of purified and cell-associated tissue factor. Thromb Res. (1988);   52(3):247-61. PMID:3194899

Note on publication

Describes the study on interaction between   factor VII and TF using a library of murine antibodies that have specificity   for TF.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.