Key features and details | |
Cat. No. | MABL-718 |
Name | Anti-CD146 mAbs |
Clone No. | AFD- ABX-MA1 (3.19.1) |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assay, immunotherapy, WB, ELISA, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds human CD146 (MCAM/MUC18). CD146 is a cell-surface glycoprotein composed of a signal peptide, five immunoglobulin-like domains, a transmembrane region, and a short cytoplasmic tail . Its expression is associated with tumor progression and the development of metastatic potential. |
Alternative Name | MCAM; MUC18; Mel-CAM; cell surface glycoprotein MUC18; cell surface glycoprotein P1H12; melanoma cell adhesion molecule; melanoma-associated antigen A32; melanoma- associated antigen MUC18; S-endo 1 endothelial-associated antigen; c3.19.1 |
UniProt | P43121 |
Immunogen | The original antibody was generated by immunizing XenoMouse® mice with SK-MEL-28 cells admixed 1:1 v/v with Complete Freund's Adjuvant (CFA), followed by four injections with 5 μg soluble MUC18-human IgG2 Fc fusion protein admixed 1:1 v/v with Incomplete Freund's Adjuvant (IFA) and then a final boost of 10 μg soluble MUC18-human IgG2 Fc fusion protein without adjuvant. |
Application Notes | A number of studies have identified MUC18 as a marker of tumor progression and metastasis in melanomas (PMID: 2602381; 3542195; 8162602). The binding specificity of the human IgG2 version of this antibody for human CD146 was checked using ELISA. The original human IgG2 version of this antibody was also able to detect MUC18 expressed on melanoma cell lines like A375SM, TXM-13 and WM- 2664 in a western blot assay. The human IgG2 version was also able to identify native MUC18 expression on A375-SM and WM-2664 cells in a flow cytometric assay (PMID: 12208768; US6924360). ABX-MA1 had no effect on melanoma cell proliferation rate in vitro. ABX-MA1 treatment of melanoma cells in vitro significantly inhibited the promoter and collagenase activity of matrix metalloproteinase 2, resulting in decreased invasion through Matrigel-coated filters. |
Antibody First Published | Mills et al. Fully human antibodies to MCAM/MUC18 inhibit tumor growth and metastasis of human melanoma. Cancer Res. 2002 Sep 1;62(17):5106-14. PMID:12208768 |
Note on publication | Studies the effect of ABX-MA1 on angiogenesis, tumor growth and metastasis. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
